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Updated: Jan 22, 2026

An Integrated Approach for Microprotein Identification and Sequence Analysis
Published on: July 12, 2022
An Approach for the Identification of Proteins Modified with ISG15
Tomoharu Takeuchi1, Shun Koinuma2, Hideyoshi Yokosawa3
1Laboratory of Biochemistry, Faculty of Pharmacy and Pharmaceutical Sciences, Josai University, Saitama, Japan. t-take@josai.ac.jp.
Abstract:
Interferon-stimulated gene 15 (ISG15) encodes a protein that is most upregulated by type I interferon stimulation and, upon activation, is conjugated to various target proteins in a process known as ISGylation. ISGylation has been shown to have roles in various biological phenomena such as viral infection and cancer. To gain further insight into the function of ISGylation, it would be useful to be able to identify ISGylated proteins. Here, we describe a method for the identification of proteins modified with ISG15. This method involves the generation of stable ISG15-transfectant cells, followed by affinity purification, and then identification of the ISGylated proteins by mass spectrometry.
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