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Differential expression profile analysis of cisplatin‑regulated miRNAs in a human gastric cancer cell line

Chunlin Yin1, Xianxian Zheng2, Heping Xiang3

  • 1Department of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. China.

Molecular Medicine Reports
|July 2, 2019
PubMed

Insights

Cisplatin, a chemotherapy drug, alters microRNA (miRNA) expression in human gastric cancer cells. These miRNA changes are linked to complex biological processes and signaling pathways, suggesting a key role in cisplatin's anticancer effects.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Cisplatin is a widely used chemotherapy agent effective against various tumors.
  • MicroRNAs (miRNAs) play crucial roles in cancer development and treatment.
  • Gastric cancer remains a significant health concern, necessitating novel therapeutic strategies.

Purpose of the Study:

  • To investigate the differential expression of miRNAs induced by cisplatin in human gastric cancer cells.
  • To identify specific miRNAs regulated by cisplatin exposure.
  • To explore the biological processes and pathways influenced by cisplatin-regulated miRNAs.

Main Methods:

  • Human gastric cancer cell line (NCI-87) treated with cisplatin.
  • High-throughput sequencing to identify differentially expressed miRNAs.
  • Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) for validation.

Main Results:

  • High-throughput sequencing identified 33 upregulated and 16 downregulated miRNAs.
  • RT-qPCR confirmed significant changes in five upregulated and five downregulated miRNAs.
  • Expression levels of hsa-miR-1246 and hsa-miR-892b were consistent with sequencing data.
  • Pathway analysis indicated that cisplatin-regulated miRNAs affect key biological processes and signaling pathways.

Conclusions:

  • Cisplatin induces significant differential expression of miRNAs in gastric cancer cells.
  • These miRNA alterations are associated with complex biological processes and signaling pathways.
  • Cisplatin likely exerts its anticancer effects in gastric cancer through these miRNA-mediated mechanisms.

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