Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

What is Gene Expression?01:42

What is Gene Expression?

195.1K
Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
195.1K
What is Gene Expression?01:36

What is Gene Expression?

11.1K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
11.1K
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

6.6K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
6.6K
Expressing Solution Concentration02:48

Expressing Solution Concentration

66.1K
A solute is a component of a solution that is typically present at a much lower concentration than the solvent. Solute concentrations are often described with qualitative terms such as dilute (of relatively low concentration) and concentrated (of relatively high concentration).
Concentrations may be quantitatively assessed using a wide variety of measurement units, each convenient for particular applications. Molarity (M) is a useful concentration unit for many applications in chemistry.
66.1K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

16.3K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.3K
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

24.7K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
24.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Spatial-temporal Evolution and Driving Force Analysis of FVC in the Arid Region of Northwest China].

Huan jing ke xue= Huanjing kexue·2025
Same author

Microbe-induced gene silencing of fungal gene confers efficient resistance against <i>Fusarium graminearum</i> in maize.

aBIOTECH·2025
Same author

Resinacein S, a novel triterpenoid from functional mushroom Ganoderma resinaceum, curbs obesity by regulating thermogenesis and energy metabolism.

Journal of food science·2025
Same author

Association Between Ankle-Brachial Index and Risk of Early Neurological Deterioration After Acute Isolated Pontine Infarction.

Brain and behavior·2025
Same author

Uncoupling protein 1 deficiency leads to transcriptomic differences in livers of pregnancy female mice and aggravates hepatic steatosis.

Archives of biochemistry and biophysics·2025
Same author

Exploring the challenges of RNAi-based strategies for crop protection.

Advanced biotechnology·2025

Related Experiment Video

Updated: Jan 22, 2026

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets
10:08

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets

Published on: November 22, 2024

1.5K

LRRFIP1 expression triggers platelet agglutination by enhancing αIIbβ3 expression.

Xiang Yin1, Peng Liu1, Yao-Yao Liu1

  • 1Department of Spinal Surgery, Institute of Surgery Research, Daping Hospital, Army Medical University, Chongqing 400042, P.R. China.

Experimental and Therapeutic Medicine
|July 2, 2019
PubMed
Summary

Leucine-rich repeat flightless-interacting protein-1 (LRRFIP1) promotes platelet agglutination. Knocking out the LRRFIP1 gene inhibits this process, suggesting LRRFIP1

Keywords:
leucine-rich repeat flightless-interacting protein-1plateletplatelet aggregationαIIbβ3

More Related Videos

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
10:34

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells

Published on: April 14, 2010

16.0K
AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain
09:59

AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain

Published on: March 31, 2022

3.2K

Related Experiment Videos

Last Updated: Jan 22, 2026

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets
10:08

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets

Published on: November 22, 2024

1.5K
Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
10:34

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells

Published on: April 14, 2010

16.0K
AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain
09:59

AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain

Published on: March 31, 2022

3.2K

Area of Science:

  • Hematology
  • Immunology
  • Molecular Biology

Background:

  • Platelets are crucial for hemostasis and host defense.
  • The role of leucine-rich repeat flightless-interacting protein-1 (LRRFIP1) in platelet function is not well understood.

Purpose of the Study:

  • To investigate the effect of LRRFIP1 on platelet agglutination.
  • To explore the potential clinical applications of LRRFIP1 in thrombotic diseases.

Main Methods:

  • Recombinant LRRFIP1 protein synthesis.
  • Generation of an LRRFIP1 gene knockout mouse model.
  • Platelet isolation and aggregation assays.
  • Flow cytometry analysis of αIIbβ3 integrin activation.

Main Results:

  • Recombinant LRRFIP1 protein successfully expressed and triggered platelet agglutination.
  • LRRFIP1 gene knockout significantly inhibited platelet agglutination and reduced αIIbβ3 levels.
  • LRRFIP1 knockout decreased αIIbβ3 levels in platelets treated with convulxin.

Conclusions:

  • LRRFIP1 plays a significant role in promoting platelet agglutination.
  • LRRFIP1 knockout inhibits platelet aggregation and affects αIIbβ3 integrin activation.
  • LRRFIP1 may have therapeutic potential for inflammatory and atherothrombotic diseases.