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Small Nucleolar RNA 71A Promotes Lung Cancer Cell Proliferation, Migration and Invasion via MAPK/ERK Pathway

Guiliang Tang1, Zihang Zeng1, Wenjie Sun1

  • 1Department of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, China.

Journal of Cancer
|July 2, 2019
PubMed

Insights

Small nucleolar RNA 71A (SNORA71A) is overexpressed in non-small cell lung cancer (NSCLC). SNORA71A promotes NSCLC progression and may be a therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Dysregulated small nucleolar RNAs (snoRNAs) are implicated in tumor development.
  • The specific role of SNORA71A in non-small cell lung cancer (NSCLC) progression was previously unclear.

Purpose of the Study:

  • To investigate the expression and function of SNORA71A in NSCLC.
  • To determine if SNORA71A could serve as a prognostic biomarker or therapeutic target for NSCLC.

Main Methods:

  • Analysis of SNORA71A expression in NSCLC tissues using GEO dataset GSE19188.
  • In vitro and in vivo loss-of-function studies (siRNA knockdown) in NSCLC cell lines and mouse xenograft models.
  • Assessment of cell proliferation, cell cycle, invasion, migration, epithelial-mesenchymal transition (EMT), and MAPK/ERK signaling pathway activity.

Main Results:

  • SNORA71A was significantly overexpressed in NSCLC tissues and associated with poorer patient survival.
  • SNORA71A knockdown inhibited NSCLC cell proliferation, induced G0/G1 phase arrest, and suppressed tumor growth in vivo.
  • Downregulation of SNORA71A reduced cell invasion, migration, EMT, and MEK/ERK phosphorylation in the MAPK/ERK pathway.

Conclusions:

  • SNORA71A acts as an oncogene in NSCLC progression.
  • SNORA71A represents a potential therapeutic target and a promising prognostic biomarker for NSCLC.
Keywords:
MAPK/ERKNon-small cell lung cancerSNORA71ASmall nucleolar RNATumorigenesis

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