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Updated: Jan 22, 2026

Optical Clearing of Plant Tissues for Fluorescence Imaging
Published on: January 5, 2022
Impact of optical tissue clearing on the Brillouin signal from biological tissue samples
Rafael J Jiménez Riobóo1, Manuel Desco2,3,4,5, María Victoria Gómez-Gaviro2,3,4
1Instituto de Ciencia de Materiales de Madrid ((ICMM), Consejo Superior de Investigaciones Científicas (CSIC), C/ Sor Juana Inés de la Cruz, 3, 28049 Madrid, Spain.
Abstract:
Brillouin spectroscopy is a well-established technology in condensed matter physics to characterize the mechanical properties of inert materials, and it has been extended very recently to the study of biological samples. Transparency is beneficial for samples to be properly analyzed by Brillouin spectroscopy. Here, we explored the efficacy of optical tissue clearing techniques to improve the acquisition of Brillouin spectra from biological tissues in order to analyze their biomechanical properties. We describe the first application of Brillouin scattering to optically cleared biological tissues with CUBIC protocol. We conclude that, within the range of error, tissue clearing does not modify the mechanical properties of the studied biological tissues.
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