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Updated: Jan 22, 2026

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

Published on: July 6, 2019

High-resolution nonlinear fluorescence microscopy using repetitive stimulated transition based on the saturation of

Fumihiro Dake, Seri Hayashi

|July 2, 2019

Abstract:

High-resolution nonlinear fluorescence (NF) microscopy that utilizes repetitive stimulated transition due to the saturation of stimulated emission caused by two-color continuous-wave lasers was developed. The resulting NF signal, detected via the lock-in technique, is produced by the multiplicative combination of incident beams, which results in an improvement of the optical resolution. The proposed method is demonstrated to have a three-dimensional optical resolution superior to that of conventional NF microscopy. The results of biological imaging reveal the feasibility and superiority of the proposed method.

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