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Updated: Jan 22, 2026

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice
Published on: January 12, 2017
Reparative Mineralized Tissue Characterization after Direct Pulp Capping with Calcium-Silicate-Based Cements
Xuan Vinh Tran1,2, Hamideh Salehi3, Minh Tam Truong4
1EA 2496, Laboratory of Orofacial Pathologies, Imaging and Biotherapies, Dental School, Paris Descartes University, Sorbonne Paris Cite, 1 rue Maurice Arnoux, 92120 Montrouge, France. vinhdentist@yahoo.com.
Mineral Trioxide Aggregate (MTA) and Biodentine promote pulp healing. Both biomaterials facilitate reparative dentin formation with structures and composition similar to natural dentin, supporting vital pulp therapy success.
Area of Science:
- Biomaterials Science
- Dental Regenerative Medicine
- Mineralogy
Background:
- Preserving dental pulp vitality is crucial in endodontic therapies.
- Treatment success hinges on clinical factors and biomaterial choice.
- Mineral Trioxide Aggregate (MTA) and Biodentine are leading pulp capping agents.
Purpose of the Study:
- To compare the reparative dentin formed after direct pulp capping with Biodentine and MTA.
- To analyze the chemical composition and microstructure of newly formed dentin.
- To assess the influence of these biomaterials on pulp healing and regeneration.
Main Methods:
- Direct pulp capping using Biodentine and MTA in experimental models.
- Scanning Electron Microscopy (SEM) for microstructural analysis of reparative dentin.
- Raman microspectroscopy for evaluating the chemical composition of dentin bridges.
Main Results:
- Reparative dentin bridges formed in both Biodentine and MTA groups.
- SEM revealed dentin tubules within the reparative dentin.
- Raman spectroscopy indicated chemical composition similar to primary dentin.
Conclusions:
- Calcium-silicate-based cements (Biodentine and MTA) create a favorable environment for pulp repair.
- The formed reparative dentin exhibits characteristics resembling primary dentin.
- These findings support the use of Biodentine and MTA in vital pulp therapies for pulp regeneration.
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