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Generation of Prostate Cancer Patient Derived Xenograft Models from Circulating Tumor Cells
Published on: October 20, 2015
Lupane Triterpenoids and New Derivatives as Antiproliferative Agents Against Prostate Cancer Cells
María Julia Castro1, Valeria Pilar Careaga2,3, Paula Alejandra Sacca3
1Institute of Chemistry of the South (INQUISUR), CONICET, Department of Chemistry, National University of the South, Bahía Blanca, Argentina julia.castro@uns.edu.ar.
Background/Aim:
This study examined the potential role of natural triterpenoids lupeol, calenduladiol and heliantriol B2, and a set of 19 derivatives, as antiproliferative and antimetastatic agents against prostate cancer cells.
Materials And Methods:
Natural triterpenoids were isolated from Chuqiraga erinaceae. Analogs were obtained by transformations of lupeol and calenduladiol. The effects of compounds on PC-3 and LNCaP cells were determined using the MTT assay. Compounds with half-maximal inhibitory concentration <70 μM were evaluated as antimetastatic agents by a wound-healing assay.
Results:
Lupeol-3β-sulfate, a new semisynthetic lupane, was the most active compound. In general, sulfated derivatives displayed higher activity than the lead against both cell lines. A new analog, calenduladiol-3β-monosulfate, inhibited the migration of PC-3 cells; heliantriol B2 and 3β-aminolupane inhibited the migration of LNCaP cells in a concentration-dependent manner.
Conclusion:
Our study provides novel agents with cytotoxic effects on prostate cancer cells, which may represent a potential new therapeutic approach for prostate cancer.
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