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Updated: Jan 22, 2026

Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina
Published on: November 10, 2017
Quantitative analysis of super-resolved structures using ASAP
John S H Danial1,2,3, Ana J Garcia-Saez4,5
1Interfaculty Institute of Biochemistry, University of Tübingen, Tübingen, Germany. js2494@cam.ac.uk.
Abstract:
Super-resolution microscopy allows imaging of cellular structures with high throughput and detail. However, the efficient and quantitative analysis of images generated is challenging with existing tools. Here, we develop ASAP (automated structures analysis program) to enable rapid and automated detection, classification and quantification of super-resolved structures. We validate ASAP on ground truth data and demonstrate its broad applicability by analyzing images of nucleoporins, TORC1 complexes, endocytic vesicles and Bax pores.
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