Related Experiment Video
Updated: Jan 22, 2026

Electroporation-mediated RNA Interference Method in Odonata
Published on: February 6, 2021
Characterization of an Amphetamine Interference from Gabapentin in an LC-HRMS Method
Ana Celia Muñoz-Muñoz1, Teresa Pekol1, Dana Schubring1
1Research and Development, Dominion Diagnostics LLC, 211 Circuit Drive, North Kingstown, RI 02852, USA.
Abstract:
An amphetamine interference was observed during the development of an liquid chromatography-high-resolution mass spectrometry (LC-HRMS) multi-class confirmation method for the determination of 47 drugs and metabolites in urine. The interference passed all qualitative criteria for amphetamine leading to potential false-positive results. Upon investigation, it was found that the amphetamine interference was correlated with the presence of high levels of gabapentin. Gabapentin is routinely detected in patient urine specimens at levels in excess of 1 mg/mL as it is widely prescribed at high doses and does not undergo significant metabolism. The source of the interference was identified as a gabapentin in-source fragment isomeric with protonated amphetamine. Here we describe the characterization of this interference and how its effect was mitigated in the LC-HRMS method.
Related Concept Videos
Interference and Diffraction
High-Resolution Mass Spectrometry (HRMS)
RNA Interference
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
CNS Stimulants: Cocaine, Amphetamines and Cannabinoids
Interference and Decay
Interference occurs when competing memories hinder the retrieval of particular information. It can be classified into two types: proactive and retroactive interference. Proactive...
Interference: Path Lengths
Two special sources may be considered when they are in phase. This can be easily achieved by feeding the two sources from the same source. An example would be synchronizing the two speakers by feeding them with the same source, such as the sound waves produced by a tuning fork. This setup ensures that the two sources have the same frequency and are...

