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OsPEX5 regulates rice spikelet development through modulating jasmonic acid biosynthesis.

Xiaoman You1, Shanshan Zhu2, Wenwei Zhang1

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Summary

OsPEX5 regulates rice spikelet development by mediating peroxisomal import of OsOPR7, an enzyme in jasmonic acid (JA) biosynthesis. This pathway is crucial for rice reproduction and grain yield.

Keywords:
OsMYC2OsPEX5jasmonic acidricespikelet development

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Area of Science:

  • Plant Biology
  • Molecular Genetics
  • Agricultural Science

Background:

  • Rice spikelet development is crucial for grain yield but poorly understood.
  • OsPEX5, a peroxisomal targeting sequence 1 (PTS1) receptor, is implicated in plant development.

Purpose of the Study:

  • To elucidate the molecular mechanisms regulating rice spikelet development.
  • To investigate the role of OsPEX5 in spikelet morphology and jasmonic acid (JA) biosynthesis.

Main Methods:

  • Genetic analysis of OsPEX5 mutants and OsOPR7 knockout mutants generated via CRISPR-Cas9.
  • Biochemical assays to confirm protein interactions and gene expression analysis.
  • Exogenous application of jasmonic acid (JA) to assess phenotypic rescue.

Main Results:

  • Mutations in OsPEX5 lead to abnormal rice spikelet morphology.
  • OsPEX5 interacts with OsOPR7, facilitating its peroxisomal import and JA biosynthesis.
  • OsOPR7 deficiency results in reduced JA levels and abnormal spikelets, partially rescued by exogenous JA.
  • OsMYC2 directly regulates downstream genes (OsMADS1, OsMADS7, OsMADS14) involved in spikelet development.

Conclusions:

  • OsPEX5 is essential for rice spikelet development via OsOPR7-mediated JA biosynthesis.
  • This study provides novel insights into JA biosynthesis regulation and its role in rice reproduction.
  • The OsPEX5-OsOPR7-JA pathway is a key regulator of rice grain yield.