Related Experiment Video

Updated: Jan 22, 2026

Isolation of the Side Population in Myc-induced T-cell Acute Lymphoblastic Leukemia in Zebrafish
09:06

Isolation of the Side Population in Myc-induced T-cell Acute Lymphoblastic Leukemia in Zebrafish

Published on: May 4, 2017

7.0K

The c-Myc-regulated miR-17-92 cluster mediates ATRA-induced APL cell differentiation

Xibao Yu1,2, Yanyun Hu2, Yifan Wu2

  • 1Department of Experimental Research, Sun Yat-sen University Cancer Center, State Key Laboratory Oncology in South China, Guangzhou, China.

Asia-Pacific Journal of Clinical Oncology
|July 3, 2019
PubMed
Abstract

Insights

The miR-17-92 microRNA cluster is abnormally expressed in acute promyelocytic leukemia (APL) and blocks cell differentiation. This cluster is regulated by c-Myc during all-trans-retinoic acid treatment.

Area of Science:

  • Hematology
  • Molecular Biology
  • Oncology

Background:

  • Acute promyelocytic leukemia (APL) treatment with all-trans-retinoic acid (ATRA) has advanced, but mechanisms remain unclear.
  • The miR-17-92 microRNA cluster impacts cell survival, proliferation, and apoptosis.
  • The role and regulation of miR-17-92 in APL are not well understood.

Purpose of the Study:

  • To investigate the expression and role of the miR-17-92 cluster in APL.
  • To explore the relationship between miR-17-92, c-Myc, and ATRA-induced differentiation in APL.

Main Methods:

  • Quantitative reverse transcription PCR (qRT-PCR) to analyze miR-17-92 expression.
  • Western blot to measure c-Myc expression.
  • Flow cytometry to assess cell differentiation via CD11b antigen expression.

Main Results:

  • miR-17-92 was upregulated in APL samples compared to healthy donors.
  • c-Myc and miR-17-92 expression decreased during ATRA-induced differentiation of NB4 cells.
  • miR-17-92 is directly regulated by c-Myc during APL cell differentiation.
  • Overexpression of miR-17-5p inhibited ATRA-induced differentiation.

Conclusions:

  • Abnormal miR-17-92 cluster expression contributes to the differentiation block in APL blast cells.
  • miR-17-92 is identified as a c-Myc target gene during ATRA-induced granulocytic differentiation.
Keywords:
acute myeloid leukemiaacute promyelocytic leukemiaall-trans-retinoic aciddifferentiationmicroRNA

More Related Videos

Differentiation of Human Pluripotent Stem Cells into Insulin-Producing Islet Clusters08:41

Differentiation of Human Pluripotent Stem Cells into Insulin-Producing Islet Clusters

Published on: June 23, 2023

4.3K
CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

2.8K

Related Concept Videos

Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.7K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
25.8K
Regulated Protein Degradation02:58

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
8.8K
pH Regulation in Cells01:28

pH Regulation in Cells

pH plays a critical role in maintaining normal cellular activities. It helps maintain the structure and function of various proteins, dictates the charge on cellular membranes, and is crucial for metabolic reactions inside the cell. Moreover, cells use the energy from the proton motive force to generate ATP.
Cytosolic pH
Under physiological conditions, the cytosolic pH is slightly more acidic than the extracellular pH. However, cells must prevent further acidification of their cytosol to...
7.6K
GTPases and their Regulation02:14

GTPases and their Regulation

Guanine nucleotide-binding proteins (G-proteins), also known as GTPases, are a superfamily of proteins that regulate many cellular processes, such as cell signaling, vesicular transport, and the regulation of cell shape and motility. Mutation or dysfunction of these proteins can lead to disease. There are around 40,000 known G-proteins that can broadly be classified into two groups ‒  small G-proteins consisting of a single domain and large multi-domain G-proteins.
Large G-proteins,...
9.8K
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
27.3K