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Updated: Jan 22, 2026
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Evaluation of Mammary Gland Development and Function in Mouse Models
Published on: July 21, 2011
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DC-SCRIPT deficiency delays mouse mammary gland development and branching morphogenesis
Chunling Tang1, Renske J E van den Bijgaart1, Maaike W G Looman1
1Radiotherapy & OncoImmunology Laboratory, Department of Radiation Oncology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, 6525 GA, Nijmegen, the Netherlands.
Developmental Biology
|July 3, 2019
Summary
The transcription regulator DC-SCRIPT (Zfp366) is crucial for normal mammary gland development. Its absence in mice led to delayed mammary gland morphogenesis, impacting development both in vivo and in vitro.
Area of Science:
- Developmental Biology
- Cancer Biology
- Genetics
Background:
- Mammary gland development involves complex morphogenesis after birth.
- Breast cancer is a leading cause of mortality in females globally.
- Loss of DC-SCRIPT (Zfp366) expression is a poor prognostic marker in estrogen receptor-positive breast cancer.
Purpose of the Study:
- To investigate the role of DC-SCRIPT in mammary gland development.
- To understand how DC-SCRIPT deficiency affects mammary gland morphogenesis in vivo and in vitro.
Main Methods:
- Utilized wild-type and DC-SCRIPT knockout mouse models.
- Analyzed mammary gland development in vivo.
- Examined mammary gland organoid branching in 3D cultures in vitro.
Main Results:
- DC-SCRIPT knockout mice displayed significant growth delays and breeding problems.
- DC-SCRIPT deficiency was observed to delay mammary gland morphogenesis in vivo.
- Loss of DC-SCRIPT dramatically impaired mammary organoid branching in vitro.
Conclusions:
- DC-SCRIPT plays a critical role in regulating mammary gland morphogenesis.
- DC-SCRIPT deficiency delays mammary gland development both in vivo and in vitro.
- DC-SCRIPT is identified as a novel modulator of mammary gland development.
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