Creating a Stable Short-term Housing Environment for Rabbits in a Cargo Van

A stable environment is a prerequisite for animal research. The absence of a suitable laboratory rabbit environment at the gyrotron facility, with the nearest housing being 6.3 miles away, made it challenging to investigate ocular damage induced in rabbit eyes due to exposure to high-frequency millimeter waves. Because rabbits are prone to transportation stress, it was vital to keep them on-site during research. Here we describe the creation of the stable environment necessary to perform reliable and reproducible animal experiments, by using a cargo van parked at the gyrotron facility. To control the interior environment, we placed a window air conditioner, humidifier, dehumidifier, and photocatalyst deodorizer inside the cargo area without altering the original configuration of the vehicle. Rabbits were housed in individual cages for a maximum of 6 d. Microbial contamination in the air was evaluated by using a passive sampling method. The average numbers of bacterial and fungal colony forming units per dish were 0.2 and 4.7, respectively, indicating that the van was as clean as a nonbarrier animal facility. The average temperature was 20.5 ^°C (range, 17.8 to 22.6 ^°C), and the average relative humidity was 49.4% (range, 36.2% to 63.2%). The concentration of ammonia was consistently below the detection limit of 0.5 ppm. Other environmental conditions were within appropriate levels. Rabbits lost 6.4% ± 2.2% (n =52) of their initial body weight during the 13- to 14-h transport but recovered the lost weight within 48 h after arrival.