Understanding diversity in oxidative status and oxidative stress: the opportunities and challenges ahead

Oxidative stress may be of profound biological relevance. In this Commentary, I discuss some key issues faced by the emerging field of oxidative stress ecology, and seek to provide interpretations and solutions. First, I show that the way in which we define oxidative stress has far-reaching implications for the interpretation of results, and that we need to distinguish between (1) a biochemical definition in terms of the molecular outcomes of oxidative stress (e.g. generation of oxidative damage) and (2) a biological definition in terms of the fitness consequences for the organism (e.g. effects on fertility). Second, I discuss the dangers of comparing different tissues and markers. Third, I highlight the need to pay more attention to the cross-talk between oxidative stress and other important physiological costs and functions; this will allow us to better understand the mechanistic basis of fitness costs. Fourth, I propose the 'redox signalling hypothesis' of life history to complement the current 'oxidative stress hypothesis' of life history. The latter states that oxidative damage underlies trade-offs because it affects traits like growth, reproduction or cell senescence. By contrast, the redox signalling hypothesis states that a trade-off between signalling and biochemical oxidative stress underlies the regulation of reactive oxygen species production and their subsequent control. Finally, I critically appraise our current knowledge of oxidative stress ecology, highlighting key research themes and providing an optimistic overview of future opportunities for the discipline to yield considerable insight into the ecological and evolutionary meaning of oxidative stress.