Accuracy of ITK-SNAP software for 3D analysis of a non-regular topography structure

To evaluate the accuracy of ITK-SNAP software for measuring volumes of a non-regular shape structure, using cone beam computed tomography (CBCT) scans, besides for developing a mathematical model to correct the software measurement error in case it existed.

A phantom made by moulding a rubber duck's head was filled with total (38,000 mm³) and partial volumes of water (7000 mm³, 14,000 mm³, 21,000 mm³, 28,000 mm³ and 35,000 mm³), which constituted the gold standards. The sound phantom and the phantom filled with different volumes of water were scanned in a Picasso Trio CBCT unit set at 80 kVp, 3.7 mA, 0.2 mm³ voxel and 12 × 8.5 cm field of view. Semi-automatic segmentation was performed with ITK-SNAP 3.0 software by two trained oral radiologists. Linear regression analyzed the relation between ITK-SNAP calculated volumes and the gold standard. Intraclass correlation coefficient was applied to analyze the reproducibility of the method. Significance level was set at 5%.

Linear regression analysis showed a significant relationship between ITK-SNAP volumes and the gold standard (F = 22,537.3, p < 0.0001), with an R² of 0.9993. The average error found was 4.7 (± 4.3) %. To minimize this error, a mathematical model was developed and provided a reduction of it. ICC revealed excellent intra-examiner agreements for both examiners 1 (ICC = 0.9991, p < 0.0001) and 2 (ICC = 0.9989, p < 0.0001). Likewise, inter-examiner agreement was excellent (ICC = 0.9991, p < 0.0001).

The software showed to be accurate for evaluating non-regular shape structures. The mathematical model developed reduced an already small error on the software's measurements.