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Related Experiment Video

Updated: Jan 22, 2026

Lab-Scale Model to Evaluate Odor and Gas Concentrations Emitted by Deep Bedded Pack Manure
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Self-build packed-bed bioreactor for rapid and effective BOD estimation.

Thi Thuy Phuong Pham1, Phuc Hoang Duy Nguyen2, Thi Thuy Van Nguyen2

  • 1Institute of Chemical Technology, Vietnam Academy of Science and Technology, 1 Mac Dinh Chi Street, District 1, Ho Chi Minh City, Vietnam. pttphuong@ict.vast.vn.

Environmental Science and Pollution Research International
|July 4, 2019
PubMed
Summary

A new biochemical oxygen demand (BOD) system uses a self-built bioreactor with natural microbes for accurate wastewater monitoring. This cost-effective method offers rapid, continuous BOD estimation with high stability and ease of use.

Keywords:
Biochemical oxygen demandBiosensorPacked-bed bioreactorSemi-continuous operating

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Area of Science:

  • Environmental Science
  • Biotechnology
  • Analytical Chemistry

Background:

  • Continuous monitoring of wastewater quality is crucial for effective treatment.
  • Traditional biochemical oxygen demand (BOD) measurements are often time-consuming and labor-intensive.
  • Developing rapid, on-site BOD estimation methods is essential for real-time process control.

Purpose of the Study:

  • To develop a simple, low-cost, and effective on-site system for continuous biochemical oxygen demand (BOD) estimation.
  • To utilize a natural microbial consortium immobilized on a porous carrier for BOD measurement.
  • To implement a novel semi-continuous operating mode for enhanced performance.

Main Methods:

  • Construction of a self-buildable packed-bed bioreactor system.
  • Immobilization of natural microbial consortia from target wastewater onto a porous carrier via adhesion.
  • Development and application of a semi-continuous operating mode with peak-type signals for BOD estimation.
  • Testing of bioreactor stability, accuracy, and performance across a range of pH levels.

Main Results:

  • Achieved an accuracy of over 95% using naturally immobilized microbial consortia.
  • The semi-continuous mode enabled continuous BOD estimation at high flow rates, overcoming electrode limitations.
  • Demonstrated stable operation for up to 22 hours continuously or 13 hours daily for several days, with low coefficients of variance (CoV) of 1.8% and 1.4% respectively.
  • Observed stable responses over 80 consecutive measurements and across a pH range of 6.5-8.5.

Conclusions:

  • The developed packed-bed bioreactor system provides a simple, rapid, and cost-effective solution for on-site BOD estimation.
  • The use of natural microbial consortia and a semi-continuous mode enhances system robustness, ease of use, and detection limits.
  • This technology is suitable for continuous monitoring of influent and effluent in wastewater treatment plants, offering reliable and stable performance.