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High-Throughput Protein Production in Yeast.

Francisco J Fernández1, Sara Gómez2, M Cristina Vega3

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Methods in Molecular Biology (Clifton, N.J.)
|July 4, 2019
PubMed
Summary

This chapter reviews high-throughput protein production pipelines using yeasts like Saccharomyces cerevisiae and Pichia pastoris. It highlights their scalability for producing isolated proteins and protein complexes from lab to industrial scales.

Keywords:
HTP protein production (HTPP)High throughput (HTP)Pichia pastorisRecombinant expressionSaccharomyces cerevisiaeYeast

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Area of Science:

  • Biotechnology and Molecular Biology
  • Microbial Fermentation and Protein Expression

Background:

  • Yeasts are single-celled fungi extensively utilized for recombinant protein production due to their rapid growth on inexpensive media.
  • Established knowledge of yeast genetics, metabolism, and cultivation facilitates their application in both academic and industrial protein manufacturing.
  • Yeasts are amenable to high-throughput screening and expression systems, enabling efficient evaluation of protein production candidates.

Purpose of the Study:

  • To provide a comprehensive overview of current high-throughput protein production pipelines in yeast systems.
  • To discuss the scalability of yeast-based protein expression from small-scale evaluations to industrial applications.
  • To identify future challenges and potential improvements in yeast-based recombinant protein production.

Main Methods:

  • Review of state-of-the-art high-throughput pipelines for protein production.
  • Focus on model yeast organisms: Saccharomyces cerevisiae and Pichia pastoris.
  • Discussion of established procedures for scaling protein expression.

Main Results:

  • Yeasts offer a robust platform for high-density cell cultivation and recombinant protein synthesis.
  • High-throughput pipelines enable efficient screening and optimization of protein expression.
  • Successful scalability of protein production from laboratory to industrial levels is demonstrated.

Conclusions:

  • High-throughput yeast expression systems, particularly in S. cerevisiae and P. pastoris, are powerful tools for recombinant protein production.
  • The inherent scalability of yeast cultivation ensures efficient transition from research to industrial manufacturing.
  • Continued innovation in yeast biotechnology is crucial for addressing future protein production challenges.