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Updated: Jan 22, 2026
08:20
Genome Engineering of Primary Human B Cells Using CRISPR/Cas9
Published on: November 3, 2020
6.9K
The Synergy between CRISPR and Chemical Engineering.
1Department of Biomedical Engineering, City University of Hong Kong, Kowloon Tong, Hong Kong.
Current Gene Therapy
|July 4, 2019
Summary
Chemical engineering advances CRISPR gene editing by controlling Cas9 activity, enhancing RNA stability, and improving delivery systems. These innovations minimize toxicity and off-target effects, enabling new biomedical research applications.
Area of Science:
- Biotechnology
- Chemical Engineering
- Molecular Biology
Background:
- CRISPR technology has rapidly advanced gene therapy and transgenic research.
- Chemical engineering plays a crucial role in CRISPR's development and application.
Purpose of the Study:
- To review how chemical engineering has improved CRISPR applications.
- To highlight new CRISPR technologies enabled by chemical engineering.
Main Methods:
- Chemical control of Cas9/dCas9 activity using synthetic substances for spatiotemporal and conditional regulation.
- Chemical modifications of sgRNA to enhance stability, binding affinity, and specificity.
- Development of nonviral and improved viral delivery vehicles for CRISPR components.
Main Results:
- Chemical control minimizes CRISPR toxicity and off-target effects, enabling complex genetic circuits.
- Modified sgRNA and Cas9 mRNA improve gene editing efficiency and reduce immunogenicity.
- Nonviral nanovectors and enhanced viral vectors overcome physiological barriers for in vivo genome editing.
Conclusions:
- Chemical engineering significantly enhances CRISPR efficiency, specificity, and safety.
- Engineering approaches enable novel CRISPR applications in biomedical research.
- Optimized delivery systems are key for effective in vivo CRISPR therapeutics.
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