Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Synthetic Biology02:55

Synthetic Biology

5.5K
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
5.5K
Self-Evaluation: Self-Enhancement and Self-Verification03:00

Self-Evaluation: Self-Enhancement and Self-Verification

5.7K
Social psychologists have documented that feeling good about ourselves and maintaining positive self-esteem is a powerful motivator of human behavior (Tavris & Aronson, 2008). In the United States, members of the predominant culture typically think very highly of themselves and view themselves as good people who are above average on many desirable traits (Ehrlinger, Gilovich, & Ross, 2005). Often, our behavior, attitudes, and beliefs are affected when we experience a threat to our...
5.7K
Bioavailability Enhancement: Drug Solubility Enhancement01:16

Bioavailability Enhancement: Drug Solubility Enhancement

229
Body:Bioavailability is a critical factor in determining a drug's effectiveness. It refers to the proportion of a drug that enters the circulation when introduced into the body and is, as a result, able to have an active effect. Enhancing bioavailability is essential for drugs with poor solubility, as it can significantly impact their therapeutic efficacy. Various methods are employed to increase the solubility of drugs, thereby enhancing their bioavailability.Micronization and nanonization are...
229
Bioavailability Enhancement: Drug Permeability Enhancement01:27

Bioavailability Enhancement: Drug Permeability Enhancement

194
Body:After oral administration, poor permeability often limits the rate at which drugs are absorbed through the intestinal epithelium. Enhancing drug permeability is crucial for effective therapy, and several strategies have been developed to overcome this challenge.One effective strategy involves the use of lipid-based formulations. These formulations enhance dissolution and solubility, targeting physiological mechanisms to increase drug absorption. This includes stimulating bile salt...
194
Opioid Analgesics: Synthetic and Semisynthetic Opioids01:15

Opioid Analgesics: Synthetic and Semisynthetic Opioids

957
Synthetic and semisynthetic opioids are pivotal in pain management and tackling opioid addiction. Semisynthetic opioids, including morphinans (morphine derivatives), oxycodone, oxymorphone, hydrocodone, and hydromorphone, have improved pharmacokinetic profiles compared to morphine. Additionally, heroin and 6-MAM (6-Monoacetylmorphine) show better CNS penetration than morphine due to heightened lipid solubility. Hydromorphone, a potent opioid, undergoes hepatic metabolism to form the active...
957
Bioavailability Enhancement: Drug Stability Enhancement and GI Retention01:05

Bioavailability Enhancement: Drug Stability Enhancement and GI Retention

203
Body:Improving a drug's stability in the gastrointestinal (GI) tract is paramount for enhancing its bioavailability and therapeutic effectiveness. Various strategies are employed to protect the drug from the harsh gastric milieu and to ensure its release and absorption at the desired site within the GI tract.Polymer coatings are one such method used to shield drugs from the stomach's acidic environment. By preventing premature drug release, these coatings improve the bioavailability of unstable...
203

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Observations of Modified Polyps and Polyp Leaves in Sea Pens (Cnidaria: Octocorallia): The Cases of <i>Ptilella</i> and <i>Pennatula</i>.

Ecology and evolution·2026
Same author

Beyond the Gold Electrode Standard: Using a Triazabutadiene-Maleimide Linker to Covalently Immobilize a Glycan-Polymer Biorecognition Element onto Carbon.

ACS applied bio materials·2026
Same author

Nanodisc-Forming Polymers for the Extraction of Membrane Proteins.

Biomacromolecules·2026
Same author

Core-block engineering enables control of ice recrystallisation inhibition in polymer nanoparticles.

Chemical science·2026
Same author

Antifreeze Glycolipid-Inspired Ice Recrystallization Inhibitors Based on End-Group Functionalized Poly(vinyl alcohol).

ACS macro letters·2026
Same author

Poly(sarcosine)-<i>block</i>-oligo(l-tryptophan) Copolymers as Noncovalent Inhibitors of Protein Aggregation.

ACS macro letters·2026

Related Experiment Video

Updated: Jan 22, 2026

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
12:55

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Published on: January 17, 2015

19.2K

Synthetically Scalable Poly(ampholyte) Which Dramatically Enhances Cellular Cryopreservation.

Trisha L Bailey, Christopher Stubbs, Kathryn Murray

    Biomacromolecules
    |July 4, 2019
    PubMed
    Summary
    This summary is machine-generated.

    A new polymeric cryopreservation enhancer significantly improves frozen cell recovery, achieving up to 88% in 2D models. This breakthrough offers a promising alternative to dimethyl sulfoxide (DMSO) for cell-based therapies.

    More Related Videos

    Scalable Nanohelices for Predictive Studies and Enhanced 3D Visualization
    08:03

    Scalable Nanohelices for Predictive Studies and Enhanced 3D Visualization

    Published on: November 12, 2014

    10.9K
    Rapid Development of Cell State Identification Circuits with Poly-Transfection
    09:21

    Rapid Development of Cell State Identification Circuits with Poly-Transfection

    Published on: February 24, 2023

    2.0K

    Related Experiment Videos

    Last Updated: Jan 22, 2026

    Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
    12:55

    Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

    Published on: January 17, 2015

    19.2K
    Scalable Nanohelices for Predictive Studies and Enhanced 3D Visualization
    08:03

    Scalable Nanohelices for Predictive Studies and Enhanced 3D Visualization

    Published on: November 12, 2014

    10.9K
    Rapid Development of Cell State Identification Circuits with Poly-Transfection
    09:21

    Rapid Development of Cell State Identification Circuits with Poly-Transfection

    Published on: February 24, 2023

    2.0K

    Area of Science:

    • Biomedical Science
    • Cell Biology
    • Cryobiology

    Background:

    • Current cryopreservation methods using dimethyl sulfoxide (DMSO) have limitations in quantitative cell recovery for various cell models.
    • DMSO requires removal post-thaw, complicating application and transfusion of cell-based therapies.
    • There is a critical need for improved cryopreservation techniques to support advancements in regenerative medicine.

    Purpose of the Study:

    • To develop and evaluate a novel polymeric cryopreservation enhancer for improved cell recovery and reduced cryoprotectant toxicity.
    • To assess the efficacy of the polymer in both 2D cell monolayers and suspension cultures.
    • To elucidate the mechanism of action of the polymeric cryoprotectant.

    Main Methods:

    • A scalable polymeric cryopreservation enhancer was synthesized from poly(methyl vinyl ether-alt-maleic anhydride).
    • Cryopreservation experiments were conducted on 2D cell monolayers and suspension cell cultures using the novel polymer, with and without DMSO.
    • Post-thaw cell recovery, membrane integrity, and growth rates were assessed and compared to conventional DMSO cryopreservation.

    Main Results:

    • The poly(ampholyte) cryopreservation enhancer achieved up to 88% recovery in 2D cell models, significantly outperforming conventional DMSO (24% recovery).
    • In suspension cryopreservation, the polymer allowed a reduction in DMSO concentration from 10 wt% to 2.5 wt%, minimizing side effects and processing time.
    • Cells cryopreserved with the polymer exhibited reduced membrane damage and enhanced post-thaw growth rates.

    Conclusions:

    • The novel polymeric cryopreservation enhancer offers a potent and scalable solution for improving frozen cell viability.
    • The polymer functions extracellularly by stabilizing cell membranes, presenting a new mechanism in cryoprotection.
    • This advancement holds significant potential for enhancing cell-based therapies and biomedical research by improving the cold chain and post-thaw cell quality.