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Related Concept Videos

Diversity of Antigen Receptors01:28

Diversity of Antigen Receptors

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Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
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Internal Receptors01:31

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Switching behavior in Bipolar Junction Transistors (BJTs) is a fundamental aspect utilized in various electronic circuits, particularly for digital logic applications like switches and amplifiers. In a typical switching circuit, a BJT alternates between cut-off and saturation modes, corresponding to the "off" and "on" states, respectively, thus behaving like an ideal switch.
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Enzyme-linked Receptors01:00

Enzyme-linked Receptors

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Related Experiment Video

Updated: Jan 22, 2026

Generation of Human Chimeric Antigen Receptor Regulatory T Cells
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Modulation of chimeric antigen receptor surface expression by a small molecule switch.

Alexandre Juillerat1, Diane Tkach2, Brian W Busser2

  • 1Cellectis Inc, 430E, 29th street, New York, NY, 10016, USA. ajuillerat@cellectis.com.

BMC Biotechnology
|July 5, 2019
PubMed
Summary

Researchers developed a novel "off-switch" for engineered Chimeric Antigen Receptor (CAR) T-cells. This system allows precise control over CAR T-cell manufacturing and function, addressing challenges in cancer and autoimmune disease therapies.

Keywords:
Cell engineeringChimeric antigen receptorSmall molecule switch

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Area of Science:

  • Immunology
  • Biotechnology
  • Cell Therapy

Background:

  • Engineered therapeutic cells, including Chimeric Antigen Receptor (CAR) T-cells, show promise for treating cancer and autoimmune diseases.
  • Expanding CAR T-cell targets beyond CD19 presents manufacturing challenges like autoactivation and T-cell fratricide.
  • Controlling CAR expression and T-cell function is crucial for effective therapeutic development.

Purpose of the Study:

  • To develop and evaluate an integrated
  • off-switch

Main Methods:

  • Engineered a novel CAR construct (SWIFF-CAR) with a self-cleaving degradation moiety.
  • Utilized a protease/protease inhibitor pair to control CAR expression.
  • Employed the small molecule Asunaprevir (ASN) for reversible control.

Main Results:

  • Demonstrated tight and reversible ex vivo control of CAR surface presentation.
  • Showcased control over CAR-induced signaling and cytolytic functions.
  • Successfully regulated engineered T-cell activity using a small molecule inhibitor.

Conclusions:

  • The SWIFF-CAR strategy offers a broadly adaptable method for CAR T-cell development.
  • This system helps overcome hurdles in CAR T-cell manufacturing.
  • The developed system functions as an integrated rheostat for CAR T-cells.