Related Experiment Video
Updated: Jan 22, 2026
08:32
Development and Assessment of Intracellular Infection Models for Staphylococcus aureus
Published on: January 17, 2025
1.2K
Complete Genome Sequence of Staphylococcus aureus Siphophage Lorac.
Antoine Marc1, Katie Cater2, Rohit Kongari1
1Center for Phage Technology, Texas A&M University, College Station, Texas, USA.
Microbiology Resource Announcements
|July 5, 2019
Summary
We sequenced the genome of Staphylococcus aureus siphophage Lorac. This temperate phage is genetically similar to the S. aureus prophage phiNM2, offering insights into phage diversity.
Area of Science:
- Microbiology
- Genomics
- Virology
Background:
- Staphylococcus aureus is a significant pathogen responsible for numerous infections.
- Bacteriophages, viruses that infect bacteria, play crucial roles in microbial ecosystems and evolution.
- Temperate phages can integrate into the bacterial genome as prophages, influencing host characteristics.
Purpose of the Study:
- To present the complete genome sequence of Staphylococcus aureus siphophage Lorac.
- To characterize Lorac as a phiETA-like temperate phage.
- To compare the genetic relatedness of Lorac to other S. aureus phages, specifically phiNM2.
Main Methods:
- Whole-genome sequencing of siphophage Lorac.
- Bioinformatic analysis of the obtained genome sequence.
- Comparative genomic analysis with related bacteriophages.
Main Results:
- The complete genome sequence of S. aureus siphophage Lorac has been determined.
- Lorac exhibits characteristics of a phiETA-like temperate phage.
- Nucleotide sequence analysis reveals high similarity between Lorac and the S. aureus prophage phiNM2.
Conclusions:
- The genome sequence of siphophage Lorac provides valuable data for understanding S. aureus phage diversity.
- The genetic similarity to phiNM2 suggests potential shared ancestry or horizontal gene transfer events.
- Further studies on Lorac can elucidate its biological role and potential applications in phage therapy or diagnostics.
Related Concept Videos
02:02Genomics
39.8K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
39.8K
02:30Genomic Imprinting and Inheritance
36.9K
Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
36.9K
03:21Genome Size and the Evolution of New Genes
9.0K
While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
9.0K
02:02Cis-regulatory Sequences
11.6K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.6K
02:16Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes
15.3K
The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...
15.3K
01:57Sanger Sequencing
773.7K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
773.7K