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Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation
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NOX1 Negatively Modulates Fibulin-5 in Vascular Smooth Muscle Cells to Affect Aortic Dissection

Xiaoping Hu1, Wanli Jiang2, Zhiwei Wang1

  • 1Department of Cardiovascular Surgery, Renmin Hospital of Wuhan University.

Biological & Pharmaceutical Bulletin
|July 5, 2019
PubMed

Insights

Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 1 (NOX1) negatively regulates Fibulin-5 expression in aortic dissection (AD). This study clarifies NOX1

Area of Science:

  • Cardiovascular Biology
  • Molecular Medicine
  • Oxidative Stress Research

Background:

  • Aortic dissection (AD) involves medial degeneration, with oxidative stress as a key factor.
  • Reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 1 (NOX1) shows protective effects in angiotensin II-induced AD, but its mechanism is unclear.
  • Fibulin-5 expression is decreased in AD patients, and its upstream regulation requires elucidation.

Purpose of the Study:

  • To investigate the relationship between NOX1 and Fibulin-5 in the context of aortic dissection (AD).
  • To elucidate the regulatory mechanism of NOX1 on Fibulin-5 expression in vascular smooth muscle cells.

Main Methods:

  • Analysis of NOX1 and Fibulin-5 expression levels in AD.
  • In vitro gain- and loss-of-function studies in vascular smooth muscle cells.
  • Pharmacological inhibition of NOX1 activity using VAS2870.

Main Results:

  • NOX1 expression was increased, while Fibulin-5 expression was decreased in AD.
  • NOX1 was found to negatively regulate Fibulin-5 expression in vascular smooth muscle cells.
  • Inhibition of NOX1 activity with VAS2870 led to an upregulation of Fibulin-5 expression.

Conclusions:

  • NOX1 acts as a negative modulator of Fibulin-5 in the pathogenesis of aortic dissection (AD).
  • Understanding the NOX1-Fibulin-5 interaction provides insights into AD mechanisms and potential therapeutic targets.
Keywords:
Fibulin-5aortic dissectionreduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 1vascular smooth muscle cell

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