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Updated: Jan 22, 2026

Fabrication of Size-Controlled and Emulsion-Free Chitosan-Genipin Microgels for Tissue Engineering Applications
Published on: April 13, 2022
Charge-conversion and ultrasound-responsive O-carboxymethyl chitosan nanodroplets for controlled drug delivery
Dong Meng1, Lu Guo1, Dandan Shi1
1Department of Ultrasound, Qilu Hospital of Shandong University, Jinan, Shandong, PR China.
Abstract:
Aim: O-carboxymethyl chitosan/perfluorohexane nanodroplets (O-CS NDs) and doxorubicin-loading O-carboxymethyl chitosan nanodroplets were synthesized and functionally tested as drug delivery system in vitro. Materials & methods: The characteristics, charge conversion, stability, cytotoxicity, ultrasound imaging ability, interaction with tumor cells of the nanodroplets and eradication on tumor cells of the doxorubicin-loaded nanodroplets were investigated. Results: O-CS NDs (below 200 nm) achieved higher tumor cellular associations at acidic pH, with great serum stability, pH-dependent charge conversion and good ultrasound imaging ability. Doxorubicin-loading O-carboxymethyl chitosan nanodroplets exhibited strong cytotoxicity on PC-3 cells with ultrasound exposure. Conclusion: These stable, safe and smart O-CS NDs may be a promising approach to improve cell interaction efficiency as an ultrasound imaging and cancer-targeting drug delivery system.
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