Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

24.7K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
24.7K
Alternative RNA Splicing02:18

Alternative RNA Splicing

4.9K
4.9K
RNA Splicing01:32

RNA Splicing

60.4K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.4K
Energy to Drive Translocation01:37

Energy to Drive Translocation

2.7K
Mitochondrial protein import is powered by two distinct energy sources: ATP hydrolysis and electrochemical potential across the inner membrane. Newly synthesized precursors are bound by cytosolic chaperones of the Hsp70 family, which guide them to the import receptors on the mitochondrial surface. Utilizing the energy of ATP hydrolysis, Hsp70 chaperones transfer these precursors to the TOM receptors on the mitochondrial outer membrane.
Generally, polypeptides are unfolded by two distinct...
2.7K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.7K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.7K
Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

3.3K
3.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

RNA-binding protein diversity and NLS arginines regulate FUS mixing in mRNA-rich compartments.

Cell reports·2026
Same author

HPF1 regulates the formation of FUS-dependent compartments by PARP1 and PARP2 activation on damaged DNA.

Nucleic acids research·2026
Same author

U2AF2 controls alternative splicing in speckle-proximal regions in an RS domain-dependent manner.

Nucleic acids research·2026
Same author

Proinsulin-Loaded Nanoparticles Suppress Insulitis and Induce Temporary Diabetes Remission.

Cells·2026
Same author

From TDP-43/RNA complex formation to disease-linked TDP-43 aggregation through a structural and cellular approach.

Nature communications·2026
Same author

mRNA recruitment by G3BP1 condensates is regulated by Caprin1 but requires G3BP1 binding to mRNA.

Scientific reports·2025

Related Experiment Video

Updated: Jan 22, 2026

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling
08:04

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling

Published on: October 8, 2019

9.1K

U2AF65 assemblies drive sequence-specific splice site recognition.

Manel Tari1, Valérie Manceau2, Jean de Matha Salone1

  • 1SABNP, Univ Evry, INSERM U1204, Université Paris-Saclay, Evry, France.

EMBO Reports
|July 5, 2019
PubMed
Summary

The splicing factors U2AF65 and CAPERα cooperatively bind SF3b155, forming RNA-driven liquid assemblies. These assemblies regulate exon skipping, impacting alternative splicing and gene expression.

Keywords:
RBM39SF3b1U2AF2liquid-liquid phase separationsplicing

More Related Videos

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
09:58

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

14.3K
Micro-drive Array for Chronic in vivo Recording: Tetrode Assembly
14:19

Micro-drive Array for Chronic in vivo Recording: Tetrode Assembly

Published on: April 22, 2009

34.1K

Related Experiment Videos

Last Updated: Jan 22, 2026

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling
08:04

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling

Published on: October 8, 2019

9.1K
Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
09:58

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

14.3K
Micro-drive Array for Chronic in vivo Recording: Tetrode Assembly
14:19

Micro-drive Array for Chronic in vivo Recording: Tetrode Assembly

Published on: April 22, 2009

34.1K

Area of Science:

  • Molecular Biology
  • RNA Biology
  • Gene Regulation

Background:

  • U2AF65 is crucial for splicing, anchoring U2 snRNP via its UHM domain interacting with SF3b155.
  • SF3b155 is a key U2 snRNP protein with a multi-ULM domain.

Purpose of the Study:

  • To investigate the cooperative binding of U2AF65 and CAPERα to SF3b155.
  • To explore the role of U2AF65's RS domain in phase separation and its regulation by RNA.
  • To determine the cellular impact of U2AF65 and CAPERα on alternative splicing.

Main Methods:

  • Cooperative binding assays to study protein interactions.
  • Liquid-liquid phase separation experiments.
  • RNA binding studies with pyrimidine-rich sequences.
  • Cellular knockdown experiments to assess splicing changes.

Main Results:

  • U2AF65 and CAPERα bind cooperatively to SF3b155's multi-ULM domain.
  • U2AF65's RS domain drives RNA-dependent liquid-liquid phase separation.
  • Knockdown of U2AF65 or CAPERα enhances cassette exon inclusion preceded by pyrimidine-rich motifs.
  • These findings support a model of RNA-guided condensate formation regulating splicing.

Conclusions:

  • Dynamic condensates of U2AF65 and CAPERα on repetitive RNA influence splice site choice.
  • Posttranslational modifications and recruited proteins may further refine splicing regulation within these condensates.
  • This study sheds light on the mechanisms of alternative splicing and gene expression control.