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MicroRNA-128/homeobox B8 axis regulates ovarian cancer cell progression

Rui Li1, Lingling Gong1, Pin Li1

  • 1Department of Obstetrics and Gynecology, Affiliated Hospital of Hebei University, Baoding, China.

Basic & Clinical Pharmacology & Toxicology
|July 5, 2019
PubMed

Insights

MicroRNA-128 (miR-128) acts as a tumor suppressor in ovarian cancer by targeting HOXB8. This finding suggests miR-128 could be a potential therapeutic target for ovarian cancer treatment.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • MicroRNA-128 (miR-128) is implicated as a tumor suppressor in various cancers, but its specific mechanism in ovarian cancer remains unclear.
  • Dysregulation of miR-128 and its targets is a key area of investigation in ovarian cancer pathogenesis.

Purpose of the Study:

  • To elucidate the functional role and molecular mechanism of miR-128 in ovarian cancer.
  • To investigate the regulatory relationship between miR-128 and homeobox B8 (HOXB8) in ovarian cancer cells.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) and Western blot analysis to measure miR-128 and HOXB8 expression.
  • Cell Counting Kit-8 assay for cell proliferation and flow cytometry for apoptosis analysis.
  • Dual-luciferase reporter assay to confirm direct targeting of HOXB8 by miR-128.

Main Results:

  • Ovarian cancer tissues and cell lines exhibited decreased miR-128 expression and increased HOXB8 expression.
  • Overexpression of miR-128 suppressed ovarian cancer cell proliferation and enhanced sensitivity to paclitaxel.
  • miR-128 was confirmed to directly target HOXB8, and HOXB8 knockdown reversed the effects of miR-128 inhibition.

Conclusions:

  • miR-128 functions as a tumor suppressor in ovarian cancer by directly targeting HOXB8.
  • The miR-128/HOXB8 axis represents a potential therapeutic strategy for ovarian cancer targeting.
Keywords:
MicroRNA-128cell proliferationhomeobox B8ovarian cancerpaclitaxel sensitivity

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