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PRDM14 and BLIMP1 control the development of chicken primordial germ cells

Yuya Okuzaki1, Hidenori Kaneoka1, Takayuki Suzuki2

  • 1Department of Biomolecular Engineering, Graduate School of Engineering, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan.

Developmental Biology
|July 5, 2019
PubMed

Insights

Chicken PRDM14 and BLIMP1 are crucial for primordial germ cell (PGC) development and self-renewal. Knockdown of these genes reduced PGC numbers, indicating their essential roles in early embryonic development.

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Genetics

Background:

  • Primordial germ cell (PGC) specification is vital for reproduction.
  • PR domain-containing protein 14 (PRDM14) and B lymphocyte-induced maturation protein 1 (BLIMP1) are key regulators in mouse PGCs.

Purpose of the Study:

  • To investigate the roles of chicken PRDM14 and BLIMP1 orthologs in PGC development.
  • To elucidate the regulatory pathways controlling PGC specification in avian embryos.

Main Methods:

  • Expression analysis of PRDM14 and BLIMP1 in chicken blastodermal cells and PGCs.
  • In vivo knockdown using replication-competent retroviral vectors expressing shRNAs.
  • Inhibition of signaling pathways (Activin, MEK) in cultured PGCs.

Main Results:

  • PRDM14 and BLIMP1 are expressed in chicken blastodermal cells and PGCs.
  • Knockdown of PRDM14 or BLIMP1 significantly reduced the number of SSEA-1 and chicken vasa homologue-positive PGCs.
  • Activin A and FGF2 signaling pathways appear to regulate PRDM14, BLIMP1, and NANOG expression.

Conclusions:

  • Chicken PRDM14 and BLIMP1 are essential for PGC development and maintenance.
  • NANOG, PRDM14, and BLIMP1 are involved in PGC self-renewal in both cultured cells and embryos.
  • Activin and FGF signaling pathways play a regulatory role in avian PGC development.

Keywords:
BLIMP1CVHChickenPRDM14Primordial germ cells

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