Magnetite nanoparticle as a support for stabilization of chondroitinase ABCI
Hossein Askaripour1, Manouchehr Vossoughi1, Khosro Khajeh2
1a Department of Chemical and Petroleum Engineering, Sharif University of Technology , Tehran , Iran.
Abstract:
Chondroitinase ABCI (cABCI) is a drug enzyme that can be used to treat spinal cord injuries. Due to low thermal stability of cABCI, this enzyme was immobilized on Fe3O4 nanoparticle to increase its thermal stability. The size and morphology, structure and magnetic property of the Fe3O4 nanoparticles were characterized by the analyses of SEM, XRD and VSM, respectively, and FTIR spectroscopy was employed to confirm the immobilization of cABCI on the surface of Fe3O4 nanoparticles. The results indicated that the optimum conditions for pH, temperature, cABCI-to-Fe3O4 mass ratio and incubation time in immobilization process were 6.5, 15 °C, 0.75 and 4.5 h, respectively, and about 0.037 mg cABCI was bound to 1 mg of Fe3O4 nanoparticles at these conditions. The value of Vmax was the same for free and immobilized cABCI, but Km value for immobilized cABCI was 1.6 times higher than that for free one. The storage stability of immobilized cABCI was significantly enhanced at low temperatures, e.g. free cABCI retained 19% of its activity after six days at -20 °C, while the immobilized one retained 96% of its activity. In vitro release of cABCI from Fe3O4 particles showed that about 94% of the enzyme was released after 6 h.
Related Concept Videos
Nuclear Stability
To hold positively charged protons together...
RNA Stability
Self-Help Support Groups
Accessibility and Cost-Effectiveness
One of the primary strengths of self-help...
Stability
The stability of an LTI system is determined by the roots of its characteristic equation, known as poles. A system is stable if it produces a bounded...
Support Reactions
The purpose of the supports is to prevent the translational motion of the system by applying an equal and opposite force and to prevent the system's rotation by applying...
Stability of structures


