Related Experiment Video
Updated: Jan 22, 2026

Isolating Nasal Olfactory Stem Cells from Rodents or Humans
Published on: August 22, 2011
Alterations in cellular force parameters and cell projections in Nasal polyps-derived fibroblasts
Jônatas Bussador do Amaral1, Antonio Carlos Bloise2, Carolina Nunes França3
1ENT Research Lab., Department of Otorhinolaryngology - Head and Neck Surgery, Federal University of Sao Paulo, Rua Coronel Lisboa, 958, Vila Clementino, São Paulo, SP, 04020-041, Brazil.
Objective:
Chronic Rhinosinusitis with Nasal Polyps (CRSwNP) is a disease that features a mechanical dysfunction involving chronic inflammation and altered tissue remodeling. In this study, we aim to evaluate the fibroblast morphology and its cellular traction force in primary fibroblasts cell cultures obtained from both healthy individuals (n=7) and patients with CRSwNP (n=8).
Methods:
Using a Traction-force Microscopy we analyzed parameters of Force/Tension in fibroblasts cultures in both experimental groups.
Results:
The analysis of the Projected Area of Cell revealed that fibroblasts derived from nasal mucosa of healthy individuals have an area on average 39.24% larger than the fibroblasts obtained from the nasal polyp tissue. We also observed that the parameters directly related to the force of the cell, Max Cumulative Force and Net Contractile Moment, presented a high Force/Tension per unit of area in the fibroblasts derived from the healthy nasal mucosa (on average 41% and 52.54% higher than the fibroblasts of the nasal polyp respectively).
Conclusion:
Our results demonstrate a cellular mechanism that may be associated with the mechanical dysfunction found in the Nasal Polyp tissue. The weak traction force of nasal polyp-derived fibroblast may, in lower dimensions, impact on the remodeling of nasal mucosa in CRSwNP.
Related Concept Videos
Fischer Projections
Newman Projections
The organic molecules rotate across the single bonds leading to numerous temporary three-dimensional structures of varying energy known as...
Alterations in Respiration II
In Biot's breathing, the respiratory rate and depth are irregular, alternating between periods of deep gasping and apnea. Common causes...
Altered States of Awareness
The ingestion of substances like stimulants or hallucinogens leads to chemical alterations in the brain...
Nose and Nasal Cavity
Electromotive Force

