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Updated: Jan 22, 2026

A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales
Published on: May 10, 2022
Multi-immersion open-top light-sheet microscope for high-throughput imaging of cleared tissues
Adam K Glaser1, Nicholas P Reder2, Ye Chen3
1Department of Mechanical Engineering, University of Washington, Seattle, WA, 98195, USA. akglaser@uw.edu.
Abstract:
Recent advances in optical clearing and light-sheet microscopy have provided unprecedented access to structural and molecular information from intact tissues. However, current light-sheet microscopes have imposed constraints on the size, shape, number of specimens, and compatibility with various clearing protocols. Here we present a multi-immersion open-top light-sheet microscope that enables simple mounting of multiple specimens processed with a variety of clearing protocols, which will facilitate wide adoption by preclinical researchers and clinical laboratories. In particular, the open-top geometry provides unsurpassed versatility to interface with a wide range of accessory technologies in the future.
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