Multi-immersion open-top light-sheet microscope for high-throughput imaging of cleared tissues

Area of Science:

• Biomedical imaging
• Microscopy techniques
• Tissue clearing

Background:

• Advances in optical clearing and light-sheet microscopy enable detailed analysis of intact tissues.
• Existing light-sheet microscopes have limitations regarding specimen size, shape, quantity, and clearing protocol compatibility.

Purpose of the Study:

• To develop a versatile light-sheet microscope addressing current limitations.
• To facilitate wider adoption of advanced tissue imaging in research and clinical settings.

Main Methods:

• Development of a multi-immersion, open-top light-sheet microscope.
• Demonstration of compatibility with various clearing protocols and multiple specimen mounting.

Main Results:

• The new microscope design simplifies the mounting of multiple specimens.
• The open-top geometry offers significant versatility for integrating future technologies.

Conclusions:

• The presented microscope overcomes previous constraints, enabling broader application of light-sheet microscopy for cleared tissues.
• This innovation is expected to accelerate structural and molecular investigations in preclinical research and clinical laboratories.