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Derivation of Cardiac Progenitor Cells from Embryonic Stem Cells
Published on: January 12, 2015
Isolation and Characterization of Cardiac Progenitor Cells
Parul Dixit1, Rajesh G Katare2
1Department of Physiology-HeartOtago, School of Biomedical Sciences, University of Otago, Dunedin, New Zealand.
Abstract:
Cardiac progenitor cells (CPCs) are gaining interest as a therapeutic option for the treatment of the heart. Due to the limited pool of CPCs residing in the heart, it is essential to isolate and expand the CPCs in vitro. Here we describe the protocol for isolation and culture of the heterogeneous population of CPCs from right atrial appendage and left ventricular tissue collected from patients undergoing on-pump coronary artery bypass graft surgery for the treatment of ischemic heart disease. Our protocol is developed to simultaneously isolate, culture, and characterize the CPCs from both atrial and ventricular tissues. We also describe the protocol for flow cytometry and immunohistochemical characterization of the isolated CPCs.
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