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Glycyrrhiza glabra-Enhanced Extract and Adriamycin Antiproliferative Effect on PC-3 Prostate Cancer Cells

Katerina Gioti1, Anastasia Papachristodoulou1,2, Dimitra Benaki2

  • 1School of Health Sciences and Education, Department of Nutrition and Dietetics, Harokopio University, Athens, Greece.

Nutrition and Cancer
|July 6, 2019
PubMed

Insights

Glycyrrhiza glabra enhanced extract (GGE), a licorice extract, demonstrates anticancer effects against prostate cancer cells. GGE, alone or with Adriamycin, inhibits cancer cell growth via apoptosis and autophagy.

Area of Science:

  • Oncology
  • Pharmacology
  • Natural Products Chemistry

Background:

  • Prostate cancer is a leading global cancer, with limited treatment options for metastatic disease.
  • Adriamycin (ADR), a chemotherapy drug, has significant side effects.
  • Natural products, like licorice (Glycyrrhiza glabra), show potential anticancer activities.

Purpose of the Study:

  • To evaluate the cytotoxic effects of Glycyrrhiza glabra enhanced extract (GGE) on prostate cancer cells (PC-3).
  • To investigate the combined effects of GGE and Adriamycin (ADR) on PC-3 cell proliferation.
  • To elucidate the mechanisms underlying GGE's anti-prostate cancer activity, including apoptosis and autophagy.

Main Methods:

  • PC-3 cells were treated with GGE and/or ADR.
  • Cell proliferation was assessed using the MTT assay.
  • Cell cycle, apoptosis, autophagy gene expression, and metabolite profiles were analyzed via flow cytometry, ELISA, Western blotting, real-time PCR, and NMR spectrometry.

Main Results:

  • GGE exhibited antiproliferative effects on PC-3 cells, both independently and in combination with ADR.
  • The study identified apoptosis and autophagy as key mechanisms mediating GGE's cytotoxic action.
  • NMR spectrometry provided insights into the specific metabolites involved in GGE's effects.

Conclusions:

  • GGE possesses significant antiproliferative and cytotoxic potential against prostate cancer PC-3 cells.
  • Co-administration of GGE with ADR may offer a more effective therapeutic strategy with potentially reduced side effects.
  • The findings highlight GGE as a promising natural compound for prostate cancer treatment, acting through apoptosis and autophagy pathways.

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