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Peritoneal cell population of mice infected with Mesocestoides corti as a source of eosinophils
Abstract:
A prominent feature of the inflammatory cellular response in the peritoneal cavity of Mesocestoides corti-infected mice is a marked and sustained increase in the number of eosinophils. In intact mice, the total number of nucleated cells in the peritoneal cavity rises from less than 5 X 10(6) to more than 50 X 10(6) and, at certain time points, in excess of 50% of these cells are eosinophils. Peritoneal eosinophils are absent in infected hypothymic nude (nu/nu) mice of three genotypes, and eosinophils counts can be elevated in infected nude mice by injection of peripheral lymphoid cells or thymocytes. The peritoneal cells of M. corti-infected mice are a convenient starting cell population for eosinophil purification.
Insights
In Mesocestoides corti-infected mice, eosinophils dramatically increase in the peritoneal cavity, with nude mice lacking this response. Lymphoid cells can restore eosinophil counts in these mice.
Area of Science:
- Immunology
- Parasitology
- Cell Biology
Background:
- Mesocestoides corti infection triggers a significant inflammatory response in the peritoneal cavity.
- Eosinophils are a major component of this inflammatory cellular infiltrate in intact mice.
Purpose of the Study:
- To investigate the role of T-cells in eosinophil recruitment during Mesocestoides corti infection.
- To characterize the peritoneal eosinophil response in immunocompetent and T-cell deficient mice.
Main Methods:
- Induction of Mesocestoides corti infection in intact and hypothymic nude mice.
- Analysis of peritoneal lavage fluid for cell counts and differential.
- Restoration of peritoneal eosinophilia by adoptive transfer of lymphoid cells or thymocytes.
Main Results:
- Intact mice showed a marked, sustained increase in peritoneal eosinophils, exceeding 50% of total nucleated cells.
- Peritoneal eosinophils were absent in infected nude (nu/nu) mice.
- Eosinophil counts were restored in infected nude mice following injection of peripheral lymphoid cells or thymocytes.
Conclusions:
- T-cells are critical for the development of peritoneal eosinophilia during Mesocestoides corti infection.
- Peritoneal eosinophils in infected mice represent a readily accessible population for purification and further study.