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Updated: Jan 22, 2026

Minimum Volume Vitrification of Immature Feline Oocytes
Published on: June 24, 2020
The Viability of Serval (Leptailurus serval) and Pallas Cat (Felis manul) Oocytes after Cryopreservation Using the
A Nowak1, J Kochan1, S Prochowska2
1University of Agriculture in Krakow, Faculty of Animal Sciences, Institute of Veterinary Sciences, al. Mickiewicza 21, 30-120 Krakow, Poland.
Background:
Vitrification by Rapid-I method could be essential for felid rescue programs to protect wild felid in the future.
Objective:
This study was aimed at adapting the Rapid I method and evaluating the viability of serval and Pallas cat oocytes compared to oocytes of the domestic cat.
Materials And Methods:
Oocytes after collection and in vitro maturation were vitrified using Cryotech medium (Cryotech, Japan) and a Rapid-I device (Vitrolife, Sweden). To evaluate viability, oocytes after warming were stained with fluorescein diacetate and ethidium bromide.
Results:
Survival rate in the control group (domestic cat) was 75 %. In the experimental group, 70% (serval) and 60% (pallas cat) viable oocytes were found.
Conclusion:
The Rapid-I method can be applied successfully for the vitrification of wild felid oocytes.
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