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Rui Ma1,2, Julien Berro1,2,3

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Area of Science:

  • Cell Biology
  • Biophysics
  • Computational Biology

Background:

  • Actin is vital for clathrin-mediated endocytosis in yeast.
  • Actin polymerization alone may not generate sufficient force to overcome yeast turgor pressure.

Purpose of the Study:

  • To investigate if crosslinking nonpolymerizing actin filaments can generate force.
  • To quantify the forces produced by actin crosslinking.

Main Methods:

  • Brownian dynamics simulations were employed.
  • A meshwork of nonpolymerizing actin filaments with crosslinkers was modeled.

Main Results:

  • Crosslinking actin filaments produces significant compressive forces.
  • Forces up to several thousand pico-Newtons were achieved with stiff crosslinkers.
  • Force generation is dependent on crosslinker stiffness and decays over time due to turnover.

Conclusions:

  • Crosslinking nonpolymerizing actin filaments is a viable mechanism for generating force in cellular processes.
  • This mechanism converts chemical binding energy into elastic energy to overcome cellular resistance.
  • The findings offer insights into the biophysical forces driving endocytosis.