Buccal rhythmogenesis and CO₂ sensitivity in Lithobates catesbeianus tadpole brainstems across metamorphosis

Bullfrog tadpoles ventilate both the buccal cavity and lung. In isolated brainstems, the midbrain/pons influences CO₂ responsiveness and timing of lung ventilatory bursting, depending on larval development. However, little is known about midbrain/pons influences on buccal burst patterns. As such, we investigated how removal of this region affects buccal burst shape and CO₂ responsiveness across development. We measured facial nerve activity in brainstems isolated from tadpoles during early and late developmental stages, under normal and elevated levels of CO₂. Brainstems were either left intact or transected by removing the midbrain/pons. In late stage preparations, buccal burst pattern differed between intact and reduced preparations, and bursts were responsive to elevated CO₂ in these reduced preparations. These results suggest the midbrain/pons affects tadpole buccal burst pattern and CO₂ responsiveness, perhaps similar to its influences on lung ventilation.