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Updated: Jan 22, 2026
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Substrate Generation for Endonucleases of CRISPR/Cas Systems
Published on: September 8, 2012
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Lactonase SsoPox modulates CRISPR-Cas expression in gram-negative proteobacteria using AHL-based quorum sensing
Sonia Mion1, Laure Plener2, Benjamin Rémy3
1Aix Marseille Univ, IRD, APHM, MEPHI, IHU-Méditerranée Infection, Marseille, France.
Research in Microbiology
|July 7, 2019
Summary
Enzymatic disruption of bacterial quorum sensing (QS) reduced CRISPR-Cas gene expression in Pseudomonas aeruginosa and Chromobacterium violaceum. This suggests QS interference can modulate phage-bacteria interactions.
Area of Science:
- Microbiology
- Bacterial Communication
- Gene Regulation
Background:
- Quorum sensing (QS) is a cell-density-dependent communication system in bacteria.
- QS regulates crucial processes like virulence, biofilm formation, and CRISPR-Cas gene regulation in proteobacteria.
Purpose of the Study:
- To investigate the impact of a quorum sensing-interfering enzyme on CRISPR-Cas systems.
- To explore the modulation of phage-bacteria interactions via QS disruption.
Main Methods:
- Utilized a quorum sensing-interfering enzyme.
- Examined CRISPR-Cas gene expression in model and clinical strains of Pseudomonas aeruginosa.
- Tested the effect on the marine bacterium Chromobacterium violaceum CV12472.
Main Results:
- CRISPR-Cas gene expression decreased in most tested bacterial strains.
- Enzymatic disruption of QS effectively altered CRISPR-Cas regulation.
Conclusions:
- Enzymatic interference with quorum sensing shows promise for modulating CRISPR-Cas systems.
- This approach offers a potential strategy for controlling phage-bacteria interactions.
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