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Updated: Jan 22, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope
Published on: October 28, 2018
An Affordable Stereomicroscope for Microsurgery Training with Fluorescence Mode.
Renan Maximilian Lovato1, Aline Lariessy Campos Paiva2, Francisco Spessatto Pesente3
1Division of Neurosurgery, Department of Surgery at Santa Casa de São Paulo School of Medical Sciences (FCMSCSP), São Paulo - SP, Brazil; Department of Neurosurgery, University Hospital of the Londrina State University, Londrina - PR, Brazil.
This study demonstrates that using a stereomicroscope with fluorescence mode enhances microsurgery training. This affordable method allows for fluorescein videoangiography, improving assessment of anastomosis patency in laboratory settings.
Area of Science:
- Neurosurgery Training
- Microsurgery Education
- Surgical Skills Development
Background:
- Laboratory training is crucial for neurosurgical skill development.
- Animal models and microscopy are traditional training tools.
- Fluorescein enhances realism and anastomosis assessment in training.
Purpose of the Study:
- To evaluate a stereomicroscope with fluorescence mode for microsurgery training.
- To assess the utility of fluorescein videoangiography in this model.
Main Methods:
- Utilized a stereomicroscope with white light and fluorescence mode.
- Performed arterial dissection and anastomosis on a chicken wing model.
- Employed fluorescein videoangiography to assess anastomosis patency.
Main Results:
- The stereomicroscope effectively substitutes for a surgical microscope in training.
- Fluorescence mode enabled high-quality fluorescein videoangiography.
- The method proved valuable for assessing anastomosis patency.
Conclusions:
- Stereomicroscopes with fluorescence are suitable for microsurgery laboratory training.
- This approach offers an affordable and reproducible method for enhancing surgical skills.
- Fluorescein videoangiography improves the quality of microsurgical training.
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