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Updated: Jan 22, 2026

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis
Published on: July 16, 2020
Membrane mimetic systems in CryoEM: keeping membrane proteins in their native environment
Henriette E Autzen1, David Julius2, Yifan Cheng3
1Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143, USA; Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus, Denmark.
Abstract:
Advances in electron microscopes, detectors and data processing algorithms have greatly facilitated the structural determination of many challenging integral membrane proteins that have been evasive to crystallization. These breakthroughs facilitate the application and development of various membrane protein solubilization approaches for structural studies, including reconstitution into lipid nanoparticles. In this review, we discuss various approaches for preparing transmembrane proteins for structural determination with single-particle electron cryo microscopy (cryoEM).
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