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A 1H NMR based study of hemolymph metabonomics in different resistant silkworms, Bombyx mori (Lepidotera), after

Xue-Yang Wang1, Zuo-Min Shao2, Ying-Jian Zhang2

  • 1Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu 212018, China; The Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture, Sericultural Research Institute, Chinese Academy of Agricultural Science, Zhenjiang, Jiangsu 212018, China.

Journal of Insect Physiology
|July 8, 2019
PubMed

Insights

This study reveals that resistant silkworms (YeA) upregulate trehalose and TCA cycle metabolites to combat Bombyx mori nucleopolyhedrovirus (BmNPV) infection, unlike susceptible strains (YeB). These metabolic shifts are key to understanding silkworm BmNPV resistance.

Area of Science:

  • Metabolomics
  • Insect Pathology
  • Biochemistry

Background:

  • Bombyx mori nucleopolyhedrovirus (BmNPV) is a significant silkworm pathogen.
  • The molecular mechanisms underlying silkworm defense against BmNPV remain largely unelucidated.

Purpose of the Study:

  • To investigate the metabolic variations in silkworm hemolymph in response to BmNPV infection.
  • To identify key metabolic pathways and metabolites involved in silkworm resistance to BmNPV.

Main Methods:

  • Comparative metabolomics using 1H NMR to analyze hemolymph metabolites.
  • Quantitative analysis of trehalose, TCA cycle intermediates, and branched-chain amino acid (BCAA) pathway metabolites.
  • Validation of metabolic enzyme expression using RT-qPCR.

Main Results:

  • Resistant silkworm strain YeA showed persistently upregulated trehalose and TCA cycle metabolites post-BmNPV infection compared to susceptible strain YeB.
  • Significant accumulation of phenylalanine and tyrosine was observed in YeA.
  • Glycolysis and gluconeogenesis pathways exhibited lower activity in YeA.

Conclusions:

  • Trehalose metabolism and the TCA cycle are critical components of the silkworm's defense response to BmNPV.
  • Metabolic profiling provides insights into the molecular basis of silkworm resistance to viral pathogens.
  • This research establishes a foundation for understanding and potentially enhancing silkworm immunity against BmNPV.
Keywords:
BmNPVBombyx mori (B. mori)MetabonomicsNMRResistant mechanism

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