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Evidence of an anti-A1 inhibited by EDTA
1Department of Medicine, Montefiore Hospital, Pittsburgh, Pennsylvania.
EDTA is added to some commercial A and B cells as a means of preventing hemolysis in serum testing. Unexpected results in serum testing have been reported with EDTA-dependent agglutinins, but not with agglutinins inhibited by EDTA. A serum sample was found to contain an anti-A1 undetected by serum testing, but resulting in incompatible crossmatches. Studies indicated that reactivity was inhibited by the EDTA present in the diluent of the A reagent red cells.
EDTA is added to some commercial A and B cells as a means of preventing hemolysis in serum testing. Unexpected results in serum testing have been reported with EDTA-dependent agglutinins, but not with agglutinins inhibited by EDTA. A serum sample was found to contain an anti-A1 undetected by serum testing, but resulting in incompatible crossmatches. Studies indicated that reactivity was inhibited by the EDTA present in the diluent of the A reagent red cells.