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Related Experiment Video

Updated: Jan 22, 2026

Innovative Strategies for Organ Preservation in Heart Transplantation: Uniform Cooling Preservation and Ex-situ Normothermic Perfusion

Innovative Strategies for Organ Preservation in Heart Transplantation: Uniform Cooling Preservation and Ex-situ Normothermic Perfusion

Published on: November 28, 2025

Systems engineering the organ preservation process for transplantation.

Reinier J de Vries¹, Martin Yarmush², Korkut Uygun³

¹Center for Engineering in Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA; Shriners Hospital for Children, Boston, MA, USA; Department of Surgery, University of Amsterdam, Amsterdam, The Netherlands.

Current Opinion in Biotechnology

|July 8, 2019

Summary

Integrating machine perfusion and subzero preservation offers a systems engineering approach to significantly extend organ preservation times. This innovation aims to enable on-demand organ availability for transplantation through advanced organ banking.

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Last Updated: Jan 22, 2026

Innovative Strategies for Organ Preservation in Heart Transplantation: Uniform Cooling Preservation and Ex-situ Normothermic Perfusion

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Published on: November 28, 2025

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Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation

Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation

Published on: July 15, 2015

Area of Science:

Organ transplantation science
Cryobiology
Biomedical engineering

Background:

Current organ preservation methods limit transplantation success and donor pool size.
Machine perfusion improves graft assessment but has limited efficacy in extending preservation duration.
Subzero preservation shows promise for significantly longer organ storage, nearing clinical application.

Purpose of the Study:

To shift the perspective from individual technologies to a systems engineering approach for organ preservation.
To integrate machine perfusion and subzero preservation strategies.
To achieve on-demand availability of donor organs through organ banking.

Main Methods:

Review of current organ preservation technologies, including machine perfusion and subzero methods.
Exploration of systems engineering principles applied to organ preservation.
Analysis of advances in cryobiology relevant to clinical translation.

Main Results:

Machine perfusion enhances graft viability assessment but has limitations in extending preservation time.
Subzero preservation techniques offer substantial potential for extending organ preservation duration.
A systems engineering approach can synergize these technologies.

Conclusions:

Integrating machine perfusion with subzero preservation via a systems engineering framework is key to advancing organ transplantation.
This integrated approach promises to overcome current limitations in organ preservation time.
The ultimate goal is to establish organ banking for on-demand organ availability, revolutionizing transplantation practices.