Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Extracellular Matrix01:42

The Extracellular Matrix

88.3K
Overview
88.3K
The Extracellular Matrix01:29

The Extracellular Matrix

12.0K
Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
12.0K
Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

8.9K
The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
8.9K
Fibril-associated Collagen01:11

Fibril-associated Collagen

3.2K
Fibril-associated collagens are a type of collagens present in the extracellular matrix with interrupted triple helices or FACIT (Fibril-associated collagens interrupted triple-helices). FACIT help connect and attach the collagen fibrils with each other as well as with other proteins of the extracellular matrix.
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
3.2K
Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

3.4K
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...
3.4K
What is Behavior?00:54

What is Behavior?

10.2K
Behaviors are actions that an organism engages in—they can be related to finding food, reproducing, defending against threats, and many other possible actions. Behaviors include activities related to the environment around the animal—such as migration—as well as social interactions within a species or population. Many behaviors involve motor output—that is, muscle movements—while others involve less visible actions, such as learning.
10.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>In situ</i> programming of CAR-macrophages <i>via</i> a polyplex nanomaterial and STING activation for triple-negative breast cancer immunotherapy.

Nanoscale·2026
Same author

Non-destructive approaches for retrieving T-cells from fibrous scaffolds for therapeutic applications.

Biomaterials science·2025
Same author

Functionalized Poly(ethylene Glycol) Diacrylate Scaffolds for <i>In Situ</i> Immunomodulation of Dendritic Cells Targeting Melanoma Tumor.

ACS biomaterials science & engineering·2025
Same author

Eugenol-Loaded Lipid Nanoparticles-Derived Hydrogels Ameliorate Psoriasis-like Skin Lesions by Lowering Oxidative Stress and Modulating Inflammation.

ACS pharmacology & translational science·2024
Same author

Surface-modified injectable poly(ethylene-glycol) diacrylate-based cryogels for localized gene delivery.

Biomedical physics & engineering express·2024
Same author

Acellular scaffold-based approach for in situ genetic engineering of host T-cells in solid tumor immunotherapy.

Military Medical Research·2024

Related Experiment Video

Updated: Jan 22, 2026

Planar Gradient Diffusion System to Investigate Chemotaxis in a 3D Collagen Matrix
09:26

Planar Gradient Diffusion System to Investigate Chemotaxis in a 3D Collagen Matrix

Published on: June 12, 2015

8.9K

Glycated collagen - a 3D matrix system to study pathological cell behavior.

Hiren Dandia1, Khushi Makkad1, Prakriti Tayalia1

  • 1Department of Biosciences & Bioengineering, Indian Institute of Technology Bombay, Mumbai, Maharashtra 400076, India. prakriti@iitb.ac.in.

Biomaterials Science
|July 9, 2019
PubMed
Summary

Diabetes and aging increase advanced glycated end products (AGEs) in collagen, altering the tumor microenvironment and promoting breast cancer cell migration and invasion via AGE-RAGE signaling.

More Related Videos

Concentric Gel System to Study the Biophysical Role of Matrix Microenvironment on 3D Cell Migration
11:43

Concentric Gel System to Study the Biophysical Role of Matrix Microenvironment on 3D Cell Migration

Published on: April 3, 2015

8.9K
Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo
10:24

Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo

Published on: May 9, 2016

17.7K

Related Experiment Videos

Last Updated: Jan 22, 2026

Planar Gradient Diffusion System to Investigate Chemotaxis in a 3D Collagen Matrix
09:26

Planar Gradient Diffusion System to Investigate Chemotaxis in a 3D Collagen Matrix

Published on: June 12, 2015

8.9K
Concentric Gel System to Study the Biophysical Role of Matrix Microenvironment on 3D Cell Migration
11:43

Concentric Gel System to Study the Biophysical Role of Matrix Microenvironment on 3D Cell Migration

Published on: April 3, 2015

8.9K
Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo
10:24

Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo

Published on: May 9, 2016

17.7K

Area of Science:

  • Biochemistry
  • Cell Biology
  • Biomaterials Science

Background:

  • Hyperglycemia, common in diabetes, and aging accelerate non-enzymatic glycosylation (glycation) of proteins.
  • This glycation forms advanced glycated end products (AGEs), leading to collagen crosslinking and altered extracellular matrix (ECM) properties.
  • Increased ECM stiffness and altered architecture are implicated in cancer progression.

Purpose of the Study:

  • To investigate the impact of glycated collagen on breast cancer cell behavior.
  • To explore the roles of matrix architecture and Receptor for Advanced Glycation End products (RAGE)-mediated signaling.
  • To develop a 3D model system for studying diabetes-related effects on the tumor microenvironment.

Main Methods:

  • Collagen type I was glycated using glucose or ribose to mimic non-enzymatic glycation.
  • MDA-MB-231 breast cancer cells were cultured within the glycated collagen matrix.
  • Cell morphology, migration, and 3D invasion were analyzed, alongside matrix properties (fiber bundling, pore size, stiffness).
  • AGE-RAGE signaling pathways were investigated.

Main Results:

  • Glycation induced collagen fiber bundling, increased matrix pore size, and enhanced stiffness.
  • AGE formation activated AGE-RAGE signaling, influencing cell morphology and migration.
  • Both matrix architecture and AGE-RAGE signaling affected 3D cell invasion.
  • The impact of AGE-RAGE signaling was more significant than altered matrix architecture alone.

Conclusions:

  • Glycated collagen, mimicking diabetic conditions, significantly alters the tumor microenvironment.
  • AGE-RAGE signaling plays a crucial role in breast cancer cell behavior within this altered matrix.
  • The developed 3D system provides a valuable tool for studying the interplay between metabolic diseases and cancer progression.