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Updated: Jan 22, 2026

Methyl-binding DNA capture Sequencing for Patient Tissues
Published on: October 31, 2016
FeII4L4 Tetrahedron Binds to Nonpaired DNA Bases
Jinbo Zhu1, Cally J E Haynes2, Marion Kieffer2
1Cavendish Laboratory, University of Cambridge , JJ Thomson Avenue , Cambridge CB3 0HE , United Kingdom.
Abstract:
A water-soluble self-assembled supramolecular FeII4L4 tetrahedron binds to single stranded DNA, mismatched DNA base pairs, and three-way DNA junctions. Binding of the coordination cage quenches fluorescent labels on the DNA strand, which provides an optical means to detect the interaction and allows the position of the binding site to be gauged with respect to the fluorescent label. Utilizing the quenching and binding properties of the coordination cage, we developed a simple and rapid detection method based on fluorescence quenching to detect unpaired bases in double-stranded DNA.
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