Related Experiment Video

Updated: Jan 22, 2026

'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens
11:31

'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens

Published on: July 8, 2011

14.0K

Bacillus Phage vB_BtS_B83 Previously Designated as a Plasmid May Represent a New Siphoviridae Genus

Emma G Piligrimova1,2, Olesya A Kazantseva3, Nikita A Nikulin3,4

  • 1Laboratory of Bacteriophage Biology, Skryabin Institute of Biochemistry and Physiology of Microorganisms, RAS, 142290 Pushchino, Russia. e.piligrimova@ibpm.ru.

Viruses
|July 10, 2019
PubMed

Insights

We identified a novel temperate Bacillus phage, B83, belonging to the Siphoviridae family. This phage, along with BMBtp14, forms a distinct viral clade, leading to the proposal of a new genus, Bembunaquatrovirus.

Area of Science:

  • Microbiology
  • Virology
  • Bacteriology

Background:

  • The Bacillus cereus group comprises species linked to human diseases and food poisoning.
  • Bacteriophages are viruses that infect bacteria and play roles in microbial ecology and evolution.

Purpose of the Study:

  • To characterize a novel temperate bacteriophage, B83, infecting the Bacillus cereus group.
  • To determine the phylogenetic placement of B83 and related phages.

Main Methods:

  • Transmission electron microscopy (TEM) for morphological analysis.
  • Comparative genomic analysis and phylogenetic reconstruction using large terminase subunits and pan-genome data.

Main Results:

  • B83, a temperate phage from the Siphoviridae family, was characterized.
  • B83 utilizes an arbitrium system for lysis-lysogeny regulation and may form a circular plasmid prophage.
  • Phylogenetic analyses revealed B83 and BMBtp14 form a distinct clade, separate from known viruses.

Conclusions:

  • B83 represents a novel temperate phage with unique characteristics.
  • The distinct phylogenetic position of B83 and BMBtp14 supports the establishment of a new genus, Bembunaquatrovirus.
Keywords:
Bacillus thuringiensisBembunaquatrovirusSiphoviridaebacteriophagephageplasmid prophage

More Related Videos

Precise Phage Mutagenesis with NgTET-Assisted CRISPR-Cas Systems10:52

Precise Phage Mutagenesis with NgTET-Assisted CRISPR-Cas Systems

Published on: October 14, 2025

628
Author Spotlight: Modeling Brain Tumors In Vivo Using Electroporation-Based Delivery of Plasmid DNA Representing Patient Mutation Signatures04:01

Author Spotlight: Modeling Brain Tumors In Vivo Using Electroporation-Based Delivery of Plasmid DNA Representing Patient Mutation Signatures

Published on: June 23, 2023

3.0K

Related Concept Videos

The Representativeness Heuristic02:13

The Representativeness Heuristic

The representative heuristic describes a biased way of thinking, in which you unintentionally stereotype someone or something. For example, you may assume that your professors spend their free time reading books and engaging in intellectual conversation, because the idea of them spending their time playing volleyball or visiting an amusement park does not fit in with your stereotypes of professors.
16.7K
Plasmids01:28

Plasmids

Plasmids are extrachromosomal DNA molecules found in bacteria, archaea, and some eukaryotic microbes like yeast. These small, circular DNA structures typically contain fewer than 30 genes, although some may exist linearly. Plasmids vary in their number within a cell, known as copy number. Single-copy plasmids are present in one copy per cell and multi-copy plasmids are present in multiple copies, reaching over 100 copies per cell.Plasmids usually replicate independently of the chromosomal DNA...
1.3K
Group Design02:01

Group Design

The most basic experimental design involves two groups: the experimental group and the control group. The two groups are designed to be the same except for one difference— experimental manipulation. The experimental group gets the experimental manipulation—that is, the treatment or variable being tested—and the control group does not. Since experimental manipulation is the only difference between the experimental and control groups, we can be sure that any differences between...
10.3K
Factorial Design02:01

Factorial Design

Factorial Analysis is an experimental design that applies Analysis of Variance (ANOVA) statistical procedures to examine a change in a dependent variable due to more than one independent variable, also known as factors. Changes in worker productivity can be reasoned, for example, to be influenced by salary and other conditions, such as skill level. One way to test this hypothesis is by categorizing salary into three levels (low, moderate, and high) and skills sets into two levels (entry level...
13.7K
Design Example: Designing a Residential Plumbing System01:25

Design Example: Designing a Residential Plumbing System

The design of residential plumbing systems requires carefully evaluating water demand, flow rates, and pressure dynamics to ensure both efficiency and reliability. The nature of water flow within pipes is defined by its Reynolds number, which classifies flow as either laminar (smooth) or turbulent.
1.1K
Design Example: Designing Water Slide01:18

Design Example: Designing Water Slide

When designing a water slide, controlling the speed of water flow is crucial for rider safety while maintaining an exciting experience. As water flows down the slide, gravity causes it to accelerate, with its speed at the bottom depending on the height from which it starts. The higher the slide, the more potential energy the water has at the top, which is converted into kinetic energy as it descends, increasing its speed.
Bernoulli's principle determines the water's velocity along the slide....
620