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Localization of the murine lambda 5 gene on chromosome 16
A Kudo1, D Pravtcheva, N Sakaguchi
1Basel Institute for Immunology, Basel, Switzerland.
Genomics
|November 1, 1987
Summary
The murine lambda 5 gene is located on chromosome 16, alongside the lambda 1 gene. This finding was determined using Southern hybridization techniques in mouse X hamster somatic cell hybrids.
Area of Science:
- Immunogenetics
- Genomics
- Molecular Biology
Background:
- The murine lambda 5 gene is part of the immunoglobulin light chain locus.
- Understanding the chromosomal location of immune system genes is crucial for studying genetic regulation and evolution.
Purpose of the Study:
- To determine the chromosomal location of the murine lambda 5 gene.
- To investigate the co-localization of the lambda 5 and lambda 1 genes.
Main Methods:
- Southern hybridization was performed on DNA from mouse X hamster somatic cell hybrids.
- Restriction enzyme digestion and probe hybridization were used to detect homologous sequences.
Main Results:
- Sequences homologous to lambda 5 DNA were identified in five out of fifteen hybrids.
- The lambda 5 gene was found on chromosome 16, the same chromosome as the lambda 1 gene.
- Analysis confirmed the germline configuration of the lambda 5 gene.
Conclusions:
- The murine lambda 5 gene is located on chromosome 16.
- The lambda 5 and lambda 1 genes are syntenic, residing on the same chromosome.