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Updated: Jan 22, 2026

Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging
Published on: December 22, 2020
Rapid MALDI mass spectrometry imaging for surgical pathology
Sankha S Basu1, Michael S Regan2, Elizabeth C Randall3
11Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115 USA.
Abstract:
Matrix assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) is an emerging analytical technique, which generates spatially resolved proteomic and metabolomic images from tissue specimens. Conventional MALDI MSI processing and data acquisition can take over 30 min, limiting its clinical utility for intraoperative diagnostics. We present a rapid MALDI MSI method, completed under 5 min, including sample preparation and analysis, providing a workflow compatible with the clinical frozen section procedure.
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