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Related Concept Videos

What is Gene Expression?01:42

What is Gene Expression?

195.1K
Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
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What is Gene Expression?01:36

What is Gene Expression?

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A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
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Cell Specific Gene Expression01:58

Cell Specific Gene Expression

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Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
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Cell Specific Gene Expression01:58

Cell Specific Gene Expression

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Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

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Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
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mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

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The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
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Related Experiment Video

Updated: Jan 22, 2026

Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads
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Analyzing magnetic bead QuantiGene® Plex 2.0 gene expression data in high throughput mode using QGprofiler.

Bie Verbist1, Eva Adriaensen2, Vikki Keersmaekers3

  • 1Janssen R&D, TMEDS, Turnhoutseweg 30, 2340, Beerse, BE, Belgium.

BMC Bioinformatics
|July 10, 2019
PubMed
Summary
This summary is machine-generated.

QGprofiler is an open-source R shiny application that streamlines QuantiGene® Plex 2.0 assay data analysis for drug discovery. It offers assay optimization, quality control, and dose-response modeling for identifying new disease-relevant chemical entities.

Keywords:
Drug discoveryHigh throughput transcriptomicsQuantigene® Plex 2.0

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Area of Science:

  • Biotechnology
  • Bioinformatics
  • Pharmacology

Background:

  • The QuantiGene® Plex 2.0 platform facilitates high-throughput gene expression analysis using bDNA and Luminex/xMAP technology.
  • Current data interpretation lacks clear instructions for quality assessment and high-throughput analysis.
  • This necessitates robust tools for accurate data processing and interpretation in drug discovery.

Purpose of the Study:

  • To introduce QGprofiler, an open-source R shiny application designed for QuantiGene® Plex 2.0 assay data analysis.
  • To provide tools for assay optimization, housekeeping gene selection, and data pre-processing up to fold change.
  • To enable dose-response modeling and cytotoxicity detection in high-throughput screening.

Main Methods:

  • Development of an R-based shiny application, QGprofiler.
  • Implementation of quality control metrics for data assessment.
  • Integration of Akaike information criterion-based model selection for dose-response analysis.

Main Results:

  • QGprofiler enables comprehensive QuantiGene® Plex 2.0 assay optimization and data pre-processing.
  • The application includes quality control metrics for robust data analysis.
  • It facilitates dose-response modeling and identification of compound cytotoxic potential.

Conclusions:

  • QGprofiler is a user-friendly, open-source application supporting drug discovery campaigns.
  • It allows testing compound libraries for dose-response effects against gene signatures.
  • The tool aids in identifying novel disease-relevant chemical entities.