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The Analysis of the Urea Biosensors Using Different Sensing Matrices via Wireless Measurement System & Microfluidic
Jung-Chuan Chou1, Cian-Yi Wu2, Si-Hong Lin2
1Graduate School of Electronic Engineering, National Yunlin University of Science and Technology, Douliu 64002, Taiwan. choujc@yuntech.edu.tw.
Sensors (Basel, Switzerland)
|July 11, 2019
Summary
This study developed two urea biosensors using magnetic beads (MBs)-urease/graphene oxide (GO)/metal oxide for remote and microfluidic detection. The nickel oxide (NiO) biosensor demonstrated superior sensitivity and linearity compared to titanium dioxide (TiO2).
Area of Science:
- Biomedical Engineering
- Materials Science
- Analytical Chemistry
Background:
- Urea detection is crucial for medical diagnostics and environmental monitoring.
- Development of sensitive and reliable urea biosensors is an ongoing research area.
- Integration of biosensors with wireless and microfluidic systems enables advanced monitoring capabilities.
Purpose of the Study:
- To develop and evaluate two types of urea biosensors integrated with wireless and microfluidic systems.
- To compare the performance of titanium dioxide (TiO2) and nickel oxide (NiO) based biosensors.
- To investigate the effect of flow rate on biosensor performance.
Main Methods:
- Fabrication of magnetic beads (MBs)-urease/graphene oxide (GO)/TiO2 and MBs-urease/GO/NiO biosensors.
- Integration of biosensors with wireless measurement and microfluidic systems.
- Characterization of sensing properties, including sensitivity and linearity, under varying flow rates.
Main Results:
- The wireless measurement system demonstrated feasibility for remote urea detection, requiring further refinement.
- The microfluidic system provided reliable urea measurements.
- The MBs-urease/GO/NiO biosensor exhibited higher average sensitivity (5.582 mV/(mg/dl)) and linearity (0.959) under dynamic conditions compared to the TiO2-based sensor.
- Sensitivity decay at high flow rates was attributed to vortex-induced vibrations (VIV).
Conclusions:
- The MBs-urease/GO/NiO biosensor offers improved performance over the TiO2-based counterpart for urea detection.
- Both wireless and microfluidic integration show promise for urea biosensing applications.
- Flow rate significantly impacts biosensor performance, necessitating optimization for dynamic environments.
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