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Quantitative immunoassay of anti-La antibodies using purified recombinant La antigen
E W St Clair1, D S Pisetsky, C F Reich
1Department of Medicine, Duke University Medical Center, Durham, NC 27710.
Arthritis and Rheumatism
|April 1, 1988
Abstract:
A purified recombinant La fusion protein was tested in an enzyme-linked immunosorbent assay to quantitate anti-La responses. This protein contained the immunodominant region of the La molecule fused to beta-galactosidase. In solid-phase assays, recombinant La protein was solubilized in urea and bound to polystyrene wells without loss of immunoreactivity. The recombinant-based enzyme-linked immunosorbent assay proved to be a sensitive method for the detection of anti-La binding, and it accurately distinguished anti-La precipitin positive sera from normal sera.